MPG Antibody

Western blot: 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the MPG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

N-Methylpurine-DNA glycosylase is encoded by the MPG gene, mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29). The completely characterized human gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of MPG to the repair of several mutagenic lesions in vivo, Hang et al.(1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of MPG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the MPG-null animals.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the C-terminus of human MPG (NKSFDQRDLAQDEAVW) was used as the immunogen for this MPG antibody.

This MPG antibody is available for research use only.

Antigen affinity

Human, Mouse, Rat

P29372