MPO Antibody Myeloperoxidase

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NSJ Bioreagents
Product Code: NSJ-R31487
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31487-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MPO antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Flow cytometry testing of human HL60 cells with MPO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPO antibody.
2 / 6
Western blot testing of human HL60 cell lysate with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).
3 / 6
Western blot testing of human 1) HL60 and 2) A431 cell lysate with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).
4 / 6
IHC staining of FFPE human spleen tissue with MPO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
IHC staining of FFPE human endometrial adenocarcinoma tissue with MPO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 6
IHC staining of FFPE human lung adenocarcinoma tissue with MPO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Flow cytometry testing of human HL60 cells with MPO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPO antibody.
Western blot testing of human HL60 cell lysate with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).
Western blot testing of human 1) HL60 and 2) A431 cell lysate with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).
IHC staining of FFPE human spleen tissue with MPO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial adenocarcinoma tissue with MPO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with MPO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the MPO antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Myeloperoxidase is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. MPO is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. Located in the nucleus as well as in the cytoplasm, intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
4353
Immunogen:
Human partial recombinant protein (AA 406-745) was used as the immunogen for this MPO antibody.
Limitation:
This MPO antibody is available for research use only.
Localization:
Nuclear and cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat