MPS1 Antibody / Metallopan-stimulin 1

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NSJ Bioreagents
Product Code: NSJ-R32561
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32561-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MPS1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of 1) rat spleen, 2) mouse liver and 3) human HeLa lysate with MPS1 antibody at 0.5ug/ml. Predicted/observed molecular weight ~10 kDa.
2 / 7
IHC testing of FFPE human intestine cancer tissue with MPS1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 7
IHC testing of FFPE mouse brain with MPS1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 7
IHC testing of FFPE rat brain with MPS1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
5 / 7
Immunofluorescent staining of FFPE human U-2 OS cells with MPS1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
6 / 7
Immunofluorescent staining of FFPE human ovarian cancer tissue with MPS1 antibody (red) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 7
Flow cytometry testing of human HeLa cells with MPS1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPS1 antibody.
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Western blot testing of 1) rat spleen, 2) mouse liver and 3) human HeLa lysate with MPS1 antibody at 0.5ug/ml. Predicted/observed molecular weight ~10 kDa.
IHC testing of FFPE human intestine cancer tissue with MPS1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE mouse brain with MPS1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat brain with MPS1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with MPS1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human ovarian cancer tissue with MPS1 antibody (red) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HeLa cells with MPS1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPS1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 1-3ug/ml,Immunocytochemistry: 1-3ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
40S ribosomal protein S27, also known as Metallopan-stimulin 1 or MPS-1, is a protein that in humans is encoded by the RPS27 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids P2-H84 from the human protein were used as the immunogen for the MPS1 antibody.
Limitation:
This MPS1 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P42677