MSH2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31697
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31697-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MSH2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of human 1) HEK293, 2) HEK293 (different lot), 3) HeLa, 4) COLO-320, 5) T-47D and 6) A549 cell lysate with MSH2 antibody. Expected molecular weight: ~105 kDa.
2 / 5
IHC-P: MSH2 antibody testing of human intestine cancer tissue. HIER: steamed with pH6 citrate buffer.
3 / 5
ICC testing of MSH2 antibody and SMMC-7721 cells
4 / 5
Immunofluorescent staining of FFPE human U-2 OS cells with MSH2 antibody (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 5
Flow cytometry testing of human A431 cells with MSH2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MSH2 antibody.
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Western blot testing of human 1) HEK293, 2) HEK293 (different lot), 3) HeLa, 4) COLO-320, 5) T-47D and 6) A549 cell lysate with MSH2 antibody. Expected molecular weight: ~105 kDa.
IHC-P: MSH2 antibody testing of human intestine cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of MSH2 antibody and SMMC-7721 cells
Immunofluorescent staining of FFPE human U-2 OS cells with MSH2 antibody (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human A431 cells with MSH2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MSH2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MSH2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MutS homolog 2 is a protein that in humans is encoded by the MSH2 gene, which is located on chromosome 2. It is a tumor suppressor gene and more specifically a caretaker gene that codes for a DNA mismatch repair (MMR) protein, MSH2 forms a heterodimer with MSH6 to make the human MutSa mismatch repair complex. It also dimerizes with MSH3 to form the MutSb DNA repair complex. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. It has been found that it may also be a coactivator of ESR1-dependent gene expression.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
4436
Immunogen:
Human partial recombinant protein (AA 337-583) was used as the immunogen for this MSH2 antibody.
Limitation:
This MSH2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity
Species Reactivity :
Human