MUC1 Antibody / Mucin-1

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NSJ Bioreagents
Product Code: NSJ-RQ4004
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4004-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MUC1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of 1) rat lung, 2) rat pancreas and 3) mouse pancreas with MUC1 antibody at 0.5ug/ml. Expected molecular weight: 120-500 kDa depending on glycosylation level.
2 / 5
IHC testing of FFPE human colon cancer tissue with MUC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 5
IHC testing of FFPE human breast cancer tissue with MUC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 5
IHC testing of FFPE human placental tissue with MUC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 5
IHC testing of FFPE mouse small intestine tissue with MUC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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Western blot testing of 1) rat lung, 2) rat pancreas and 3) mouse pancreas with MUC1 antibody at 0.5ug/ml. Expected molecular weight: 120-500 kDa depending on glycosylation level.
IHC testing of FFPE human colon cancer tissue with MUC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human breast cancer tissue with MUC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human placental tissue with MUC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse small intestine tissue with MUC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MUC1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Mucin 1, cell surface associated (MUC1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in humans. This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. It is mapped to 1q22. Mucin 1 is a transmembrane mucin normally expressed on the apical borders of secretory epithelial cells. Overexpression of Mucin 1 is often associated with colon, breast, ovarian, lung and pancreatic cancers. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Mucin 1 stimulated ESR1-mediated transcription and contributed to estradiol-mediated growth and survival of breast cancer cells. This gene also can suppress pulmonary innate immunity, and its antiinflammatory activity may play an important modulatory role during microbial infection.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human partial protein corresponding to amino acids S1062-V1139 was used as the immunogen for the MUC1 antibody.
Limitation:
This MUC1 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P15941