Myeloperoxidase Antibody MPO

Contact us
NSJ Bioreagents
Product Code: NSJ-R30233
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30233-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The lyophilized Myeloperoxidase antibody can be stored at 40. After reconstitution, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
IHC staining of FFPE mouse spleen tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 7
IHC staining of FFPE rat lymph tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human tonsil tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
Western blot testing of 1) human HL60, 2) rat thymus, 3) mouse spleen and 4) mouse thymus tissue with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).
5 / 7
Immunofluorescent staining of FFPE human spleen tissue with Myeloperoxidase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
6 / 7
Immunofluorescent staining of FFPE human appendicitis tissue with Myeloperoxidase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
7 / 7
Flow cytometry testing of human HL60 cells with Myeloperoxidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Myeloperoxidase antibody.
❮❯

IHC staining of FFPE mouse spleen tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lymph tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HL60, 2) rat thymus, 3) mouse spleen and 4) mouse thymus tissue with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).
Immunofluorescent staining of FFPE human spleen tissue with Myeloperoxidase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human appendicitis tissue with Myeloperoxidase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Flow cytometry testing of human HL60 cells with Myeloperoxidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Myeloperoxidase antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 1-2ug/ml
Application Note:
Titration of the pAb may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Myeloperoxidase is a mammalian phagocyte hemoprotein though to primarily mediate host defense reactions. It is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of the human protein (KNNIFMSNSYPRDFV) was used as the immunogen for this Myeloperoxidase antibody.
Limitation:
This Myeloperoxidase antibody is available for research use only.
Localization:
Nuclear and cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P05164