Myosin Antibody Skeletal Slow

NSJ Bioreagents
Product Code: NSJ-R30056
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30056-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: NOQ7.5.4D, aka IML-64
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rabbit
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The Myosin antibody Skeletal Slow can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Myosin antibody and rat skeletal muscle tissue lysate
2 / 3
Western blot testing of 1) monkey heart, 2) monkey skeletal muscle, 3) rat heart, 4) rat skeletal muscle, 5) mouse heart and 6) mouse skeletal muscle tissue lysate with Myosin antibody.
3 / 3
IHC staining of FFPE rat skeletal muscle tissue with Myosin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of Myosin antibody and rat skeletal muscle tissue lysate
Western blot testing of 1) monkey heart, 2) monkey skeletal muscle, 3) rat heart, 4) rat skeletal muscle, 5) mouse heart and 6) mouse skeletal muscle tissue lysate with Myosin antibody.
IHC staining of FFPE rat skeletal muscle tissue with Myosin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Further Information

Application Details :
Western blot: 0.5-2ug/ml,IHC (Paraffin): 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Myosin antibody Skeletal Slow may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. Skeletal Myosin(slow),, also known as light chain 3(MYL3), mapped to 3p. Fodor et al.(1989) found that the MYL3 gene has 7 exons, the last of which is completely untranslated 3-prime sequence.
Format :
Ascites
Formulation :
0.1mg/ml with 1% BSA and 0.01% sodium azide
Gene ID #:
4625
Immunogen:
Human skeletal muscle myosin purified from myofibrils was used as the immunogen for this Myosin antibody (Skeletal Slow).
Limitation:
This Myosin antibody Skeletal Slow is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, mouse, rat, rabbit