Myosin Phosphatase Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R30766
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30766-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Myosin Phosphatase antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of human 1) HeLa, 2) Jurkat, 3) HEK293, 4) COS-7 (monkey), 5) Raji, 6) K562, 7) Caco-2 and 8) HepG2 lysate with Myosin Phosphatase antibody. Expected molecular weight: 110~130 kDa.
2 / 4
Western blot testing of 1) rat brain, 2) rat C6, 3) mouse liver and 4) mouse NIH3T3 lysate with Myosin Phosphatase antibody. Expected molecular weight: 110~130 kDa.
3 / 4
Flow cytometry testing of human U-251 MG cells with Myosin Phosphatase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Myosin Phosphatase antibody.
4 / 4
Flow cytometry testing of human HeLa cells with Myosin Phosphatase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Myosin Phosphatase antibody.
❮❯

Western blot testing of human 1) HeLa, 2) Jurkat, 3) HEK293, 4) COS-7 (monkey), 5) Raji, 6) K562, 7) Caco-2 and 8) HepG2 lysate with Myosin Phosphatase antibody. Expected molecular weight: 110~130 kDa.
Western blot testing of 1) rat brain, 2) rat C6, 3) mouse liver and 4) mouse NIH3T3 lysate with Myosin Phosphatase antibody. Expected molecular weight: 110~130 kDa.
Flow cytometry testing of human U-251 MG cells with Myosin Phosphatase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Myosin Phosphatase antibody.
Flow cytometry testing of human HeLa cells with Myosin Phosphatase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Myosin Phosphatase antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Myosin Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PPP1R12A, also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in 2 ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human Myosin Phosphatase (MKMADAKQKRNEQLKRW) was used as the immunogen for this Myosin Phosphatase antibody (100% homologous in human, mouse and rat).
Limitation:
This Myosin Phosphatase antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O14974