NAMPT Antibody / Visfatin

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NSJ Bioreagents
Product Code: NSJ-R32446
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32446-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Prior to reconstitution, store at 40. After reconstitution, the Visfatin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of 1) rat brain, 2) mouse heart and 3) human placenta lysate with Visfatin antibody at 0.5ug/ml. Expected molecular weight ~56 kDa.
2 / 4
IHC testing of FFPE human breast cancer tissue with Visfatin antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
3 / 4
IHC testing of FFPE mouse intestine with Visfatin antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
4 / 4
IHC testing of FFPE rat intestine with Visfatin antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
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Western blot testing of 1) rat brain, 2) mouse heart and 3) human placenta lysate with Visfatin antibody at 0.5ug/ml. Expected molecular weight ~56 kDa.
IHC testing of FFPE human breast cancer tissue with Visfatin antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE mouse intestine with Visfatin antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE rat intestine with Visfatin antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,ELISA: 0.1-0.5ug/ml (human protein tested); request BSA-free format for coating
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme that in humans is encoded by the PBEF1 gene. This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids L62-N337 were used as the immunogen for the Visfatin antibody.
Limitation:
This Visfatin antibody is available for research use only.
Localization:
Predominantly cytoplasmic, some nuclear staining may also be seen
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P43490