NEDD8 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32590
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32590-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the NEDD8 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human A431 cells with NEDD8 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC testing of FFPE human lung cancer tissue with NEDD8 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 5
IHC testing of FFPE human lung cancer tissue with NEDD8 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 5
Flow cytometry testing of human A431 cells with NEDD8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NEDD8 antibody.
5 / 5
Western blot testing of 1) rat testis, 2) mouse thymus, 3) mouse brain, 4) human HeLa and 5) human MCF7 lysate with NEDD8 antibody at 0.5ug/ml. Predicted molecular weight ~9 kDa.
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Immunofluorescent staining of FFPE human A431 cells with NEDD8 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human lung cancer tissue with NEDD8 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human lung cancer tissue with NEDD8 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A431 cells with NEDD8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NEDD8 antibody.
Western blot testing of 1) rat testis, 2) mouse thymus, 3) mouse brain, 4) human HeLa and 5) human MCF7 lysate with NEDD8 antibody at 0.5ug/ml. Predicted molecular weight ~9 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the NEDD8 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
NEDD8 is a protein that in humans is encoded by the NEDD8 gene. Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The most substrates of NEDD8 modification are the Cullin subunits of Cullin-based E3 ubiquitin ligases, which are active only when neddylated. Their NEDDylation is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 20-60 (TDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYK-human) were used as the immunogen for the NEDD8 antibody.
Limitation:
This antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15843