Netrin 1 Antibody

Western blot: 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the Netrin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Netrin-1 (NTN1), also known as NTN1L, is a diffusible protein made by floor plate cells. It is found in the floor plate and neuroepithelial cells of the ventral region of the spinal cord, as well as other locations in the nervous system including the somatic mesoderm, pancreas and cardiac muscle. The human NTN1 gene is mapped to chromosome 17p13-p12 by FISH. Netrin-1 can attract spinal commissural axons and repel trochlear axons in vitro. And expression confers a selective advantage for tumor cell survival. The binding of netrin-1 to DCC appears to depend on the presence of a coreceptor or accessory proteins. Adenosine A2b receptor is actually its receptor and induces cAMP accumulation on binding netrin-1, and that dependent outgrowth of dorsal spinal cord axons directly involves A2b. Netrin-1 can promote intestinal tumor development, probably by regulating cell survival. Thus, a receptor or receptors function as conditional tumor suppressors.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Amino acids 32-51 (AGQAAQPDPCSDENGHPRRC) were used as the immunogen for this Netrin 1 antibody (100% homologous in human, mouse and rat).

This Netrin 1 antibody is available for research use only.

Antigen affinity

Human, Mouse, Rat

O95631