Neurofilament 200 Antibody (Heavy)
| Code | Size | Price |
|---|
| NSJ-R30062-100ug | 100 ug | £535.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: N52, aka NF-200
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Aliquot and store the Neurofilament 200 antibody at -200. Avoid repeated freezing and thawing.
Documents
Further Information
Application Details :
Western blot: 0.5ml,IHC (Paraffin): 1-2ug/ml,Immunohistochemistry (Frozen): 1-2ug/ml; formalin or acetone fixed tissues
Application Note:
Titration of the Neurofilament 200 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68kD (NFL), 125kD (NFM), and 200kD (NFH) on SDS-gel electrophoresis. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Genomic clones for the largest human neurofilament protein were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. Mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease(CMT).
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
4744
Immunogen:
C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200 was used as the immunogen for this Neurofilament 200 antibody.
Limitation:
This Neurofilament 200 antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, mouse, rat