Neurofilament Antibody (Light)

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NSJ Bioreagents
Product Code: NSJ-R30061
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30061-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: NR4, aka NF-68
Regulatory Status: RUO
Target Species:
  • Human
  • Porcine (pig)
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Neurofilament antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE mouse brain tissue with Light Chain Neurofilament antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE rat brain tissue with Light Chain Neurofilament antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of 1) human SH-SY5Y, 2) human U-251, 3) rat brain and 4) mouse brain tissue lysate with Light Chain Neurofilament antibody. Predicted molecular weight: 62-68 kDa.
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IHC staining of FFPE mouse brain tissue with Light Chain Neurofilament antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Light Chain Neurofilament antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human SH-SY5Y, 2) human U-251, 3) rat brain and 4) mouse brain tissue lysate with Light Chain Neurofilament antibody. Predicted molecular weight: 62-68 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,IHC (Paraffin): 2-4ug/ml,Immunohistochemistry (Frozen): 2-4ug/ml; formalin or acetone fixed tissues
Application Note:
The stated application concentrations are suggested starting points. Titration of the Neurofilament antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Neurofilaments are composed of three neuron-specific proteins with apparent molecular masses of 68kD (NFL), 125kD (NFM) and 200kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. NF68, also called Neurofilament Protein Light (NFL) is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E(CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
4747
Immunogen:
Pig spinal cord was used as the immunogen for this light Neurofilament antibody.
Limitation:
This Neurofilament antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, pig, rat