Neutrophil Elastase Antibody

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NSJ Bioreagents
Product Code: NSJ-R32526
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32526-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Neutrophil Elastase antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) mouse bone and 2) mouse spleen lysate with Neutrophil Elastase antibody at 0.5ug/ml. Expected molecular weight: 29-35 kDa.
2 / 3
IHC testing of FFPE mouse spleen with Neutrophil Elastase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 3
IHC testing of FFPE rat spleen with Neutrophil Elastase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of 1) mouse bone and 2) mouse spleen lysate with Neutrophil Elastase antibody at 0.5ug/ml. Expected molecular weight: 29-35 kDa.
IHC testing of FFPE mouse spleen with Neutrophil Elastase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat spleen with Neutrophil Elastase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Neutrophil elastase, also called Elane, is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids S27-N265 from the mouse protein were used as the immunogen for the Neutrophil Elastase antibody.
Limitation:
This Neutrophil Elastase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
Q3UP87