NFATC3 Antibody / NFAT4

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NSJ Bioreagents
Product Code: NSJ-R32932
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32932-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the NFAT4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of human 22RV1 cell lysate with NFAT4 antibody at 0.5ug/ml. Predicted molecular weight: ~116 kDa but can be observed at 140~150 kDa due to phosphorylation.
2 / 5
Flow cytometry testing of human SiHa cells with NFAT4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.
3 / 5
Flow cytometry testing of human U-2 OS cells with NFAT4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.
4 / 5
Flow cytometry testing of human K562 cells with NFAT4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.
5 / 5
Flow cytometry testing of human A431 cells with NFAT4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.
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Western blot testing of human 22RV1 cell lysate with NFAT4 antibody at 0.5ug/ml. Predicted molecular weight: ~116 kDa but can be observed at 140~150 kDa due to phosphorylation.
Flow cytometry testing of human SiHa cells with NFAT4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.
Flow cytometry testing of human U-2 OS cells with NFAT4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.
Flow cytometry testing of human K562 cells with NFAT4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.
Flow cytometry testing of human A431 cells with NFAT4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT4 antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA (recombinant human protein): 0.1-0.5ug/ml (BSA-free format available)
Application Note:
Optimal dilution of the NFAT4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Nuclear factor of activated T-cells, cytoplasmic 3 is a protein that in humans is encoded by the NFATC3 gene. The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids K630-L712 was used as the immunogen for the NFAT4 antibody.
Limitation:
This NFAT4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q12968