NKCC1 Antibody SLC12A2

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NSJ Bioreagents
Product Code: NSJ-R31290
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31290-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the NKCC1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of NKCC1 antibody and HeLa cell lysate. Expected/observed size ~130KD
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IHC-P: NKCC1 antibody testing of human intestinal cancer tissue
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IHC-P: NKCC1 antibody testing of rat intestine tissue
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IHC-F testing of NKCC1 antibody and rat intestine tissue
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Western blot testing of NKCC1 antibody and HeLa cell lysate. Expected/observed size ~130KD
IHC-P: NKCC1 antibody testing of human intestinal cancer tissue
IHC-P: NKCC1 antibody testing of rat intestine tissue
IHC-F testing of NKCC1 antibody and rat intestine tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the NKCC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Solute carrier family 12 (sodium/potassium/chloride transporters), member 2, also known as NKCC1 (Na-K-Cl Cotransporter 1), is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. NKCC1 is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human NKCC1 (RIDHYRHTAAQLGEKLLRP) was used as the immunogen for this NKCC1 antibody (100% homologous in human, mouse and rat).
Limitation:
This NKCC1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P55011