NLRP3 Antibody (NALP3)

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NSJ Bioreagents
Product Code: NSJ-R30750
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30750-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the NLRP3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of NLRP3 antibody and Lane 1: HEP-2; 2: A549; 3: U87; 4: CEM cell lysate
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IHC-P: NLRP3 antibody testing of human tonsil tissue
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IF/ICC testing of human THP1 cells with NLRP3 antibody at 2ug/ml.
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IF/ICC testing of human THP1 cells with NLRP3 antibody at 2ug/ml.
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Flow cytometry testing of human THP1 cells with NLRP3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NLRP3 antibody.
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Flow cytometry testing of human U937 cells with NLRP3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NLRP3 antibody.
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Immunofluorescent staining of FFPE rat colon tissue with NLRP3 antibody (red) and DAPI (nuclear counterstain, blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Immunofluorescent staining of FFPE rat spleen tissue with NLRP3 antibody (red) and DAPI (nuclear counterstain, blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Immunofluorescent staining of FFPE rat kidney tissue with NLRP3 antibody (red) and DAPI (nuclear counterstain, blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of NLRP3 antibody and Lane 1: HEP-2; 2: A549; 3: U87; 4: CEM cell lysate
IHC-P: NLRP3 antibody testing of human tonsil tissue
IF/ICC testing of human THP1 cells with NLRP3 antibody at 2ug/ml.
IF/ICC testing of human THP1 cells with NLRP3 antibody at 2ug/ml.
Flow cytometry testing of human THP1 cells with NLRP3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NLRP3 antibody.
Flow cytometry testing of human U937 cells with NLRP3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NLRP3 antibody.
Immunofluorescent staining of FFPE rat colon tissue with NLRP3 antibody (red) and DAPI (nuclear counterstain, blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of FFPE rat spleen tissue with NLRP3 antibody (red) and DAPI (nuclear counterstain, blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of FFPE rat kidney tissue with NLRP3 antibody (red) and DAPI (nuclear counterstain, blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence/Immunocytochemistry: 2-3ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the NLRP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NOD-like receptor family pyrin domain containing 3, also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 gene. The gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD(ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the proteins nucleotide-binding domain reduced ATP binding, Caspase-1 activation, IL-1b production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for NLRP3 inflammasomes in antifungal immunity, Gross et al.showed that deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human NLRP3 (RYLEDLEDVDLKKFKMHLED) was used as the immunogen for this NLRP3 antibody.
Limitation:
This NLRP3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q96P20