Nod1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31707
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31707-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Nod1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Nod1 antibody and Lane 1: human A549; 2: rat heart lysate. Predicted/observed size ~107KD
2 / 5
IHC-P: Nod1 antibody testing of human placenta tissue
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ICC testing of human SMMC-7721 cells with Nod1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 5
ICC testing of human HeLa cells with Nod1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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ICC testing of human A549 cells with Nod1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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Western blot testing of Nod1 antibody and Lane 1: human A549; 2: rat heart lysate. Predicted/observed size ~107KD
IHC-P: Nod1 antibody testing of human placenta tissue
ICC testing of human SMMC-7721 cells with Nod1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
ICC testing of human HeLa cells with Nod1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
ICC testing of human A549 cells with Nod1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Nod1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Nucleotide-binding oligomerization domain-containing protein 1, also known as CARD4, is a protein receptor that in humans is encoded by the NOD1 gene. It is a member of NOD-like receptor protein family and is a close relative of Nod2. It recognizes bacterial molecules and stimulates an immune reaction. Nod1 contains a caspase recruitment domain (CARD). This gene is an intracellular pattern recognition receptor, which is similar in structure to resistant proteins of plants, and mediates innate and acquired immunity by recognizing bacterial molecules containing D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety. Additionally, it has been shown that Nod1 can sense cytosolic microbial products by monitoring the activation state of small Rho GTPases.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
10392
Immunogen:
Human partial recombinant protein (AA 1-160) was used as the immunogen for this Nod1 antibody.
Limitation:
This Nod1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat