NOX2 Antibody / gp91phox

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NSJ Bioreagents
Product Code: NSJ-RQ4016
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4016-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the gp91phox antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) U-87 MG, 2) HeLa and 3) HepG2 cell lysate with NOX2 antibody at 0.5ug/ml. Predicted molecular weight ~65 kDa, can be observed at ~85 kDa.
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Western blot testing of human 1) U-87 MG, 2) HeLa and 3) HepG2 cell lysate with NOX2 antibody at 0.5ug/ml. Predicted molecular weight ~65 kDa, can be observed at ~85 kDa.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the gp91phox antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
NOX2 (NADPH Oxidase 2), also called CYBB (Cytochrome b(-245), beta subunit), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein. NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human partial protein corresponding to amino acids F416-D500 was used as the immunogen for the gp91phox antibody.
Limitation:
This gp91phox antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P04839