NOX4 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31029
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31029-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the NOX4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of NOX4 antibody and rat kidney lysate. Expected molecular weight: ~65 kDa, 75-80 kDa.
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Western blot testing of NOX4 antibody human 1) HepG2, 2) SW620, 3) HK-2, 4) HL-60, 5) 293T, 6) SW579 and 6) SK-OV-3 cell lysate. Expected molecular weight: ~65 kDa, 75-80 kDa.
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IHC-P: NOX4 antibody testing of rat kidney tissue
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Flow cytometry testing of human U-2 OS cells with NOX4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOX4 antibody.
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Western blot testing of NOX4 antibody and rat kidney lysate. Expected molecular weight: ~65 kDa, 75-80 kDa.
Western blot testing of NOX4 antibody human 1) HepG2, 2) SW620, 3) HK-2, 4) HL-60, 5) 293T, 6) SW579 and 6) SK-OV-3 cell lysate. Expected molecular weight: ~65 kDa, 75-80 kDa.
IHC-P: NOX4 antibody testing of rat kidney tissue
Flow cytometry testing of human U-2 OS cells with NOX4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOX4 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the NOX4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NADPH oxidase 4, also called Renal NADPH oxidase or RENOX, is an enzyme that in humans is encoded by the NOX4 gene, and a member of the NOX family of NADPH oxidases. Geiszt et al.(2000) stated that the nucleotide sequence of NOX4 matches that found in a genomic clone on chromosome 15. In a note added in proof, they stated that genomic clones assigned to chromosome 11 also contain sequence corresponding to NADPH Oxidase 4. By FISH, Shiose et al.(2001) mapped the gene to chromosome 11q14.2-q21. Geiszt et al.(2000) found that NIH 3T3 fibroblasts overexpressing the transfected protein showed increased production of superoxide and developed signs of cellular senescence. They suggested that NOX4, as a renal source of ROS, may fulfill the function of the putative oxygen sensor in the kidney. By biochemical analysis of endogenous renal NOX4, Shiose et al.(2001) determined that the enzyme can use either NADH or NADPH as an electron donor for superoxide production.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of mouse NADPH oxidase 4 (NRNNSYGTKFEYNKES) was used as the immunogen for this NOX4 antibody (100% rat homology).
Limitation:
This NOX4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9JHI8