Nucleophosmin Antibody (NPM1)

NSJ Bioreagents
Product Code: NSJ-R31031
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31031-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The lyophilized Nucleophosmin antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freeze/thaws.

Images

1 / 5
Immunofluorescent staining of FFPE human A431 cells with Nucleophosmin antibody. HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC-P: Nucleophosmin antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
3 / 5
ICC testing of Nucleophosmin antibody and HeLa cells
4 / 5
IHC-P: Nucleophosmin antibody testing of rat spleen tissue. HIER: steamed with pH6 citrate buffer.
5 / 5
Western blot testing of Nucleophosmin antibody and Lane 1:  rat testis;  2: rat brain;  3: human HeLa;  4: human U87 MG;  5: human A549;  6: human SMMC-7721 cell lysate.  Expected molecular weight: ~38 kDa.

Immunofluorescent staining of FFPE human A431 cells with Nucleophosmin antibody. HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: Nucleophosmin antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of Nucleophosmin antibody and HeLa cells
IHC-P: Nucleophosmin antibody testing of rat spleen tissue. HIER: steamed with pH6 citrate buffer.
Western blot testing of Nucleophosmin antibody and Lane 1:  rat testis;  2: rat brain;  3: human HeLa;  4: human U87 MG;  5: human A549;  6: human SMMC-7721 cell lysate.  Expected molecular weight: ~38 kDa.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
Variations in secondary/substrate sensitivities and test protocols may require the Nucleophosmin antibody to be titrated for optimal performance.
Description:
Nucleophosmin, also known as NPM, is a protein that in humans is encoded by the NPM1 gene. The gene maps to chromosome 5q35. Nucleophosmin is likely involved in the assembly of ribosomal proteins into ribosomes. An electron microscopic study indicated that Nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human NPM1 (ELKADKDYHFKVDNDENEHQ) was used as the immunogen for this Nucleophosmin antibody (100% homologous in human, mouse and rat).
Limitation:
This Nucleophosmin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P06748