Oct-1 Antibody (POU2F1)

NSJ Bioreagents
Product Code: NSJ-R31730
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31730-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Oct-1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Oct-1 antibody and Lane 1: rat liver; and human samples 2: placenta; 3: Jurkat; 4: HeLa; 5: A549; 6: SMMC-7721. Predicted/observed size ~89KD~
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IHC-P: Oct-1 antibody testing of human tonsil tissue

Western blot testing of Oct-1 antibody and Lane 1: rat liver; and human samples 2: placenta; 3: Jurkat; 4: HeLa; 5: A549; 6: SMMC-7721. Predicted/observed size ~89KD~
IHC-P: Oct-1 antibody testing of human tonsil tissue

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Oct-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
POU domain class 2 transcription factor 1, also known as OTF1 or Oct-1, is a protein that in humans is encoded by the POU2F1 gene. The transcription factor was among the first identified members of the POU transcription factor family. It has been found that the Oct-1 dimer formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1 , whereas dimers formed on the consensus MORE (more PORE) (ATGCATATGCAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) failed to interact. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
5451
Immunogen:
Human partial recombinant protein (AA 11-240) was used as the immunogen for this Oct-1 antibody.
Limitation:
This Oct-1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human