OPA1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31823
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31823-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the OPA1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with OPA1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC testing of FFPE human lung cancer tissue with OPA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE mouse liver with OPA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 7
IHC testing of FFPE rat kidney with OPA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
5 / 7
Western blot testing of human 1) MCF7, 2) PANC-1, 3) PC-3 and 4) T-47D lysate with OPA1 antibody. Predicted molecular weight: 111-120 kDa with multiple smaller isoforms from 81-95 kDa.
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Western blot testing of 1) rat brain, 2) mouse brain, 3) mouse heart and 4) mouse NIH 3T3 lysate with OPA1 antibody. Predicted molecular weight: 111-120 kDa with multiple smaller isoforms from 81-95 kDa.
7 / 7
Flow cytometry testing of human U-2 OS cells with OPA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OPA1 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with OPA1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human lung cancer tissue with OPA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse liver with OPA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat kidney with OPA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of human 1) MCF7, 2) PANC-1, 3) PC-3 and 4) T-47D lysate with OPA1 antibody. Predicted molecular weight: 111-120 kDa with multiple smaller isoforms from 81-95 kDa.
Western blot testing of 1) rat brain, 2) mouse brain, 3) mouse heart and 4) mouse NIH 3T3 lysate with OPA1 antibody. Predicted molecular weight: 111-120 kDa with multiple smaller isoforms from 81-95 kDa.
Flow cytometry testing of human U-2 OS cells with OPA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OPA1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the OPA1 antibody should be determined by the researcher.
Description:
Dynamin-like 120 kDa protein, mitochondrial is a protein that in humans is encoded by the OPA1 gene. It is mapped to 3q29. This protein regulates mitochondrial fusion and cristae structure in the inner mitochondrial membrane (IMM) and contributes to ATP synthesis and apoptosis. This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids EDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEA of human OPA1 were used as the immunogen for the OPA1 antibody.
Limitation:
This OPA1 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O60313