OPN Antibody

NSJ Bioreagents
Product Code: NSJ-R30503
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30503-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the OPN antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of OPN antibody and Lane 1: MM231; 2: HeLa; 3: Jurkat; 4: COLO320 cell lysate. The glycoprotein is visualized between 44~75.KD~
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IHC-P: OPN antibody testing of human breast cancer tissue

Western blot testing of OPN antibody and Lane 1: MM231; 2: HeLa; 3: Jurkat; 4: COLO320 cell lysate. The glycoprotein is visualized between 44~75.KD~
IHC-P: OPN antibody testing of human breast cancer tissue

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the OPN antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Osteopontin is also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrcellular matrix by osteoblasts during bone development. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human Osteopontin (EEKQLYNKYPDAVATWLNPD) was used as the immunogen for this OPN antibody.
Limitation:
This OPN antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P10451