Optineurin Antibody

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NSJ Bioreagents
Product Code: NSJ-R31756
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31756-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Optineurin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human adenosquamous carcinoma of the gall bladder with Optineurin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE mouse brain tissue with Optineurin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE rat brain tissue with Optineurin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Flow cytometry testing of human A431 cells with Optineurin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Optineurin antibody.
5 / 5
Western blot testing of 1) human HeLa, 2) human HT1080, 3) human HepG2, 4) rat heart, 5) rat brain, 6) mouse heart and 7) mouse brain tissue lysate with Optineurin antibody. Expected molecular weight: 66-74 kDa depending on phosphorylation level.
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IHC staining of FFPE human adenosquamous carcinoma of the gall bladder with Optineurin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Optineurin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Optineurin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human A431 cells with Optineurin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Optineurin antibody.
Western blot testing of 1) human HeLa, 2) human HT1080, 3) human HepG2, 4) rat heart, 5) rat brain, 6) mouse heart and 7) mouse brain tissue lysate with Optineurin antibody. Expected molecular weight: 66-74 kDa depending on phosphorylation level.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Optineurin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
OPTN is also known as NRP, FIP2 or HYPL. This gene encodes the coiled-coil containing protein Optineurin which may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize TNFa or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. It may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, Huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
10133
Immunogen:
Human partial recombinant protein (AA 241-577) was used as the immunogen for this Optineurin antibody.
Limitation:
This Optineurin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q96CV9