p27KIP1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31499
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31499-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the p27KIP1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of p27KIP1 antibody and Lane 1: rat thymus; 2: rat brain lysate. Expected/observed size ~27KD
2 / 7
Flow cytometry testing of human SiHa cells with p27KIP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p27KIP1 antibody.
3 / 7
IHC staining of FFPE human tonsil with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human tonsil with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human liver cancer with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE rat brain with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
7 / 7
ICC staining of FFPE human U-2 OS cells with p27KIP1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of p27KIP1 antibody and Lane 1: rat thymus; 2: rat brain lysate. Expected/observed size ~27KD
Flow cytometry testing of human SiHa cells with p27KIP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p27KIP1 antibody.
IHC staining of FFPE human tonsil with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat brain with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
ICC staining of FFPE human U-2 OS cells with p27KIP1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunocytochemistry (FFPE): 2-4ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the p27KIP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cyclin-dependent kinase inhibitor 1B, also known as KIP1 or P27, is an enzyme inhibitor that in humans is encoded by the CDKN1B gene. It encodes a protein which belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. p27KIP1 can inhibit both CDK activation and the kinase activity of assembled and activated cyclin-CDK. The function of the protein is associated with an aggressive phenotype in human breast cancer. Downregulation of p27KIP1 by CK2-alpha-prime is necessary for development of agonist- and stress-induced cardiac hypertrophy.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
1027
Immunogen:
Human partial recombinant protein (AA 10-198) was used as the immunogen for this p27KIP1 antibody.
Limitation:
This p27KIP1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat