p300 Antibody (KAT3B)

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NSJ Bioreagents
Product Code: NSJ-R31592
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31592-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the p300 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human COLO-320, 2) rat PC-12 and 3) mouse NIH 3T3 cell lysate with p300 antibody. Predicted molecular weight ~300 kDa.
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Western blot testing of p300 antibody and recombinant human protein (0.5ng)
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IHC-P: p300 antibody testing of rat intestine tissue
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IHC-P: p300 antibody testing of mouse intestine tissue
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IHC-P staining of human oesophagus squama cancer tissue
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Western blot testing of 1) human COLO-320, 2) rat PC-12 and 3) mouse NIH 3T3 cell lysate with p300 antibody. Predicted molecular weight ~300 kDa.
Western blot testing of p300 antibody and recombinant human protein (0.5ng)
IHC-P: p300 antibody testing of rat intestine tissue
IHC-P: p300 antibody testing of mouse intestine tissue
IHC-P staining of human oesophagus squama cancer tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the p300 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
E1A binding protein p300 is a protein that in humans is encoded by the EP300 gene. The gene is located on the long (q) arm of the human chromosome 22 at position 13.2. This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. p300 appears to be critical for normal development before and after birth. It carries out its function by activating transcription. In addition, the protein functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. p300 also mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
2033
Immunogen:
Human partial recombinant protein (AA 2065-2414) was used as the immunogen for this KAT3B / p300 antibody.
Limitation:
This p300 antibody is available for research use only.
Localization:
Intracellular
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat