p50 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31569
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31569-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The p50 antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeat freeze/thaws.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with p50 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) Raji, 2) SH-SY5Y, 3) HeLa and 4) Jurkat cell lysate with p50 antibody. Expected molecular weight: 50 kDa / 105 kDa.
3 / 3
Flow cytometry testing of human HeLa cells with p50 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p50 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with p50 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) Raji, 2) SH-SY5Y, 3) HeLa and 4) Jurkat cell lysate with p50 antibody. Expected molecular weight: 50 kDa / 105 kDa.
Flow cytometry testing of human HeLa cells with p50 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p50 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Titration of the p50 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Nuclear factor NF-kappa-B p105/p50, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NFKB1 is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NFKB1 proteins by p105 and generation of p50 by a cotranslational processing.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
4790
Immunogen:
Human partial recombinant protein (AA 1-360) was used as the immunogen for this p50 antibody.
Limitation:
This p50 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat