p63 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31167
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31167-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the p63 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with p63 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC-P: p63 antibody testing of human esophageal squamous cell carcinoma tissue
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IHC-P: p63 antibody testing of human esophageal squamous cell carcinoma tissue
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IHC-P testing of human lung cancer tissue
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ICC testing of A549 cells
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Western blot testing of 1) rat heart and 2) mouse thymus lysate (50ug/lane) with p63 antibody. Expected molecular weight: 63-77 kDa.
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Western blot testing of human 1) HeLa, 2) SMMC-7721, 3) COLO-320, 4) A549 and 5) SGC-7901 cell lysate with p63 antibody. Expected molecular weight: 63-77 kDa.
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Flow cytometry testing of human A431 cells with p63 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p63 antibody.
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Immunofluorescent staining of FFPE human A431 cells with p63 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: p63 antibody testing of human esophageal squamous cell carcinoma tissue
IHC-P: p63 antibody testing of human esophageal squamous cell carcinoma tissue
IHC-P testing of human lung cancer tissue
ICC testing of A549 cells
Western blot testing of 1) rat heart and 2) mouse thymus lysate (50ug/lane) with p63 antibody. Expected molecular weight: 63-77 kDa.
Western blot testing of human 1) HeLa, 2) SMMC-7721, 3) COLO-320, 4) A549 and 5) SGC-7901 cell lysate with p63 antibody. Expected molecular weight: 63-77 kDa.
Flow cytometry testing of human A431 cells with p63 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p63 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the p63 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Tumor Protein p63, also known as KET, is a protein that in humans is encoded by the TP63 gene. Yang et al.(1998) described the cloning of tumor protein, which shows strong homology to the tumor suppressor p53 and the p53-related protein p73. By fluorescence in situ hybridization, Yang et al.(1998) localized the human gene to chromosome 3q27-q29. Hibi et al.(2000) stated that p53 homologs known variously as p40, p51, p63, and p73L (Trink etal., 1998, Yang etal., 1998, Osada etal., 1998, Senoo etal., 1998) are isoforms of the same gene, which were then referred to as AIS for 'amplified in squamous cell carcinoma.'
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 82-98 (DMDARRNKQQRIKEEGE) were used as the immunogen for this p63 antibody.
Limitation:
This p63 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9H3D4