p65 Antibody NF-kB

Contact us
NSJ Bioreagents
Product Code: NSJ-R30754
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30754-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the p65 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with p65 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
IHC testing of FFPE human breast cancer tissue with p65 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 3
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Caco-2, 4) human Raji, 5) rat PC-12, 6) rat C6, 7) mouse NIH 3T3 and 8) mouse ANA-1 cell lysate with p65 antibody. Expected molecular weight ~65 kDa.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with p65 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human breast cancer tissue with p65 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Caco-2, 4) human Raji, 5) rat PC-12, 6) rat C6, 7) mouse NIH 3T3 and 8) mouse ANA-1 cell lysate with p65 antibody. Expected molecular weight ~65 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the p65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
RELA, also called NFKB3 or NFKB, p65 subunit is part of the NF-kB complex. The complex is inhibited by I-kappa-B proteins, which inactivates NFkB by trapping it in the cytoplasm. The p65 heterodimer is the most abundant form of NF-kB. It is a nonhistone substrate of HDAC3 and that IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes for subsequent NF-kB responses. Its nucleocytoplasmic redistribution coincided with export of PPARG, and immunoprecipitation analysis indicated that PPARG-RELA association was dependent on the PPARG C-terminal ligand-binding domain. IKK-dependent phosphorylation of RELA on Ser468 enhanced binding of GCN5 to RELA and its ubiquitination.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of the human protein (VPIEEQRGDYDLNAVR) was used as the immunogen for this p65 antibody (100% homologous in human, mouse and rat).
Limitation:
This p65 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q04206