Parkin Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R31720
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31720-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Parkin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of Parkin antibody and Lane 1: U87; 2: mouse brain lysate. Predicted molecular weight: 50-60 kDa.
2 / 5
IHC-P: Parkin antibody testing of mouse brain tissue. HIER: steamed with pH6 citrate buffer.
3 / 5
IHC-P: Parkin antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
4 / 5
IHC-P: Parkin antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
5 / 5
ICC testing of Parkin antibody and mouse Neuro-2a cells
❮❯

Western blot testing of Parkin antibody and Lane 1: U87; 2: mouse brain lysate. Predicted molecular weight: 50-60 kDa.
IHC-P: Parkin antibody testing of mouse brain tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: Parkin antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: Parkin antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of Parkin antibody and mouse Neuro-2a cells

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Parkin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Parkin is a RING domain-containing E3 ubiquitin ligase involved in proteasome-dependent degradation of proteins. It is mapped to 6q26. This gene is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy. Parkin is expressed in neuronal processes and cell bodies of neurons, but not glial cells, in the midbrain, basal ganglia, cerebral cortex, and cerebellum. Parkin assimilated with actin filaments, suggesting that it is a cytoskeletal-associated protein. Parkin is identified as a transcriptional repressor of p53 independent of its ubiquitin ligase function. It also has been found that parkin was associated physically with mitochondrial DNA (mtDNA) in proliferating as well as in differentiated SH-SY5Y neuroblastoma cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
5071
Immunogen:
Human partial recombinant protein (AA 23-416) was used as the immunogen for this Parkin antibody.
Limitation:
This Parkin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat