PARN Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4308
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4308-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PARN antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) placenta, 3) COLO-320, 4) HepG2, 5) PANC-1, 6) SGC-7901, 7) MBA-MD-231, 8) rat kidney, 9) mouse heart and 10) mouse kidney with PARN antibody at 0.5ug/ml. Predicted molecular weight ~73 kDa.
2 / 6
IHC testing of FFPE human breast cancer tissue with PARN antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 6
IHC testing of FFPE mouse small intestine tissue with PARN antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 6
IHC testing of FFPE rat small intestine tissue with PARN antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 6
IF/ICC staining of FFPE human A549 cells with PARN antibody (green) at 2ug/ml and DAPI nuclear stain (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
6 / 6
Flow cytometry testing of human A431 cells with PARN antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PARN antibody.
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Western blot testing of human 1) HeLa, 2) placenta, 3) COLO-320, 4) HepG2, 5) PANC-1, 6) SGC-7901, 7) MBA-MD-231, 8) rat kidney, 9) mouse heart and 10) mouse kidney with PARN antibody at 0.5ug/ml. Predicted molecular weight ~73 kDa.
IHC testing of FFPE human breast cancer tissue with PARN antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse small intestine tissue with PARN antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat small intestine tissue with PARN antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IF/ICC staining of FFPE human A549 cells with PARN antibody (green) at 2ug/ml and DAPI nuclear stain (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human A431 cells with PARN antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PARN antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence/Immunocytochemistry: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PARN antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Poly(A)-specific ribonuclease (PARN), also known as polyadenylate-specific ribonuclease or deadenylating nuclease (DAN), is an enzyme that in humans is encoded by the PARN gene. The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids M1-Y301 was used as the immunogen for the PARN antibody.
Limitation:
This PARN antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O95453