PARP Antibody

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NSJ Bioreagents
Product Code: NSJ-R31722
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31722-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PARP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC-P: PARP antibody testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.
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IHC-P: PARP antibody testing of human placenta tissue. HIER: steamed with pH6 citrate buffer.
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ICC testing of PARP antibody and A549 cells
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Western blot testing of human 1) HeLa, 2) HepG2, 3) COLO-320, 4) Jurkat, 5) rat PC-12, 6) mouse NIH 3T3 and 7) mouse HEPA 1-6 lysate with PARP antibody at 0.5ug/ml. Predicted molecular weight: ~116 kDa (full length), ~89 kDa (C-terminal catalytic domain).
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IHC-P: PARP antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
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IHC-P: PARP antibody testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: PARP antibody testing of human placenta tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of PARP antibody and A549 cells
Western blot testing of human 1) HeLa, 2) HepG2, 3) COLO-320, 4) Jurkat, 5) rat PC-12, 6) mouse NIH 3T3 and 7) mouse HEPA 1-6 lysate with PARP antibody at 0.5ug/ml. Predicted molecular weight: ~116 kDa (full length), ~89 kDa (C-terminal catalytic domain).
IHC-P: PARP antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the PARP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Poly [ADP-ribose] polymerase 1 is composed of four domains: a DNA-binding domain, a caspase-cleaved domain, an auto-modification domain, and a catalytic domain. In vivo, PARP is cleaved by caspases -3 and -7 between aspartic acid 214 and glycine 215, resulting in a 24KD subunit (containing the DNA binding zinc finger motif) and a 89KD subunit (containing the catalytic and auto-modification domains). PARP is an enzyme that modifies nuclear proteins. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
142
Immunogen:
Human partial recombinant protein (AA 670-858) was used as the immunogen for this PARP antibody.
Limitation:
This PARP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat