PBK Antibody

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NSJ Bioreagents
Product Code: NSJ-R31723
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31723-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The lyophilized PBK antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freeze/thaws.

Images

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Western blot testing of PBK antibody and Lane 1: rat testis; 2: mouse testis; 3: human placenta; 4: (h) Jurkat lysate. Predicted/observed size ~36KD
2 / 6
IHC-P: PBK antibody testing of human breast tissue
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IHC-P: PBK antibody testing of mouse testis tissue
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IHC-P: PBK antibody testing of rat testis tissue
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IF/ICC staining of FFPE human U-2 OS cells with PBK antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human A549 cells with PBK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PBK antibody.
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Western blot testing of PBK antibody and Lane 1: rat testis; 2: mouse testis; 3: human placenta; 4: (h) Jurkat lysate. Predicted/observed size ~36KD
IHC-P: PBK antibody testing of human breast tissue
IHC-P: PBK antibody testing of mouse testis tissue
IHC-P: PBK antibody testing of rat testis tissue
IF/ICC staining of FFPE human U-2 OS cells with PBK antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with PBK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PBK antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Variations in secondary/substrate sensitivities and test protocols may require the PBK antibody to be titrated for optimal performance.
Description:
PDZ binding kinase (PBK), also known as TOPK, is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. PBK can mediate cell growth through histone H3 modification. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. It may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
55872
Immunogen:
Human partial recombinant protein (AA 71-322) was used as the immunogen for this PBK antibody.
Limitation:
This PBK antibody is available for research use only.
Localization:
Nuclear and cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat