PCNA Antibody

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NSJ Bioreagents
Product Code: NSJ-R32371
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32371-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PC10
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PCNA antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of PCNA antibody (clone PC10) and 1) MCF-7, 2) HeLa, 3) HT1080 and 4) COLO320 cell lysate. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.
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IHC testing of FFPE human rectal cancer with PCNA antibody (clone PC10). Boiling sections in pH6 citrate buffer, followed by cooling, is required for FFPE tissue testing.
3 / 3
ICC testing of HeLa cells with PCNA antibody (clone PC10).
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Western blot testing of PCNA antibody (clone PC10) and 1) MCF-7, 2) HeLa, 3) HT1080 and 4) COLO320 cell lysate. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.
IHC testing of FFPE human rectal cancer with PCNA antibody (clone PC10). Boiling sections in pH6 citrate buffer, followed by cooling, is required for FFPE tissue testing.
ICC testing of HeLa cells with PCNA antibody (clone PC10).

Documents

Further Information

Application Details :
Western blot: 2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the PCNA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
5111
Immunogen:
PCNA recombinant protein was used as the immunogen for this PCNA antibody.
Limitation:
This PCNA antibody is available for research use only.
Purity:
Unpurified ascites
Species Reactivity :
Human, Mouse, Rat, Chicken