PDE4D Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4277
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4277-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PDE4D antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of human HeLa cell lysate with PDE4D antibody at 0.5ug/ml. Predicted molecular weight ~91 kDa.
2 / 6
IHC testing of FFPE human placental tissue with PDE4D antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 6
IHC testing of FFPE mouse lung tissue with PDE4D antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 6
IHC testing of FFPE rat lung tissue with PDE4D antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 6
Flow cytometry testing of human HeLa cells with PDE4D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=PDE4D antibody.
6 / 6
Flow cytometry testing of human A549 cells with PDE4D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=PDE4D antibody.
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Western blot testing of human HeLa cell lysate with PDE4D antibody at 0.5ug/ml. Predicted molecular weight ~91 kDa.
IHC testing of FFPE human placental tissue with PDE4D antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE mouse lung tissue with PDE4D antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat lung tissue with PDE4D antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human HeLa cells with PDE4D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=PDE4D antibody.
Flow cytometry testing of human A549 cells with PDE4D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=PDE4D antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PDE4D antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
cAMP-specific 3',5'-cyclic phosphodiesterase 4D is an enzyme that in humans is encoded by the PDE4D gene. This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids H466-Q709 was used as the immunogen for the PDE4D antibody.
Limitation:
This PDE4D antibody is available for research use only.
Localization:
Cell membrane, cytoplasm
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q08499