PDK1 Antibody

NSJ Bioreagents
Product Code: NSJ-R31100
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31100-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PDK1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of PDK1 antbody on Lane 1: rat heart; 2: (r) skeletal muscle; and human samples 3: HeLa; 4: M231; 5: COLO320; 6: SW620 cell lysate. Expected size 58~68KD~
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IHC-P: PDK1 antibody testing of human prostatic cancer tissue

Western blot testing of PDK1 antbody on Lane 1: rat heart; 2: (r) skeletal muscle; and human samples 3: HeLa; 4: M231; 5: COLO320; 6: SW620 cell lysate. Expected size 58~68KD~
IHC-P: PDK1 antibody testing of human prostatic cancer tissue

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PDK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Pyruvate dehydrogenase kinase isoenzyme 1, is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Pyruvate dehydrogenase kinase isoenzyme 1 (WKHYNTNHEADDWC) was used as the immunogen for this PDK1 antibody.
Limitation:
This PDK1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
Q15118