Perilipin Antibody / PLIN1

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NSJ Bioreagents
Product Code: NSJ-R32951
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32951-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the PLIN1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of 1) rat liver, 2) mouse liver and 3) human HepG2 lysate with PLIN1 antibody at 0.5ug/ml. Predicted molecular weight ~56 kDa.
2 / 3
Flow cytometry testing of human U-2 OS cells with PLIN1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=PLIN1 antibody.
3 / 3
Flow cytometry testing of human A431 cells with PLIN1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=PLIN1 antibody.
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Western blot testing of 1) rat liver, 2) mouse liver and 3) human HepG2 lysate with PLIN1 antibody at 0.5ug/ml. Predicted molecular weight ~56 kDa.
Flow cytometry testing of human U-2 OS cells with PLIN1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=PLIN1 antibody.
Flow cytometry testing of human A431 cells with PLIN1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=PLIN1 antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the PLIN1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Perilipin, also known as lipid droplet-associated protein or PLIN, is a protein that, in humans, is encoded by the PLIN gene. The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids DKVLGAALAGCELAWGVARDTAEFAANTRAGRLAS were used as the immunogen for the PLIN1 antibody.
Limitation:
This PLIN1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O60240