Periostin Antibody

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NSJ Bioreagents
Product Code: NSJ-R32897
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32897-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Periostin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of 1) rat stomach, 2) rat lung, 3) rat NRK cells, 4) mouse heart, 5) mouse intestine and 6) mouse lung lysate with Periostin antibody at 0.5ug/ml. Predicted molecular weight ~93 kDa.
2 / 7
IHC testing of FFPE mouse stomach tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 7
IHC testing of FFPE mouse lung tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 7
IHC testing of FFPE mouse small intestine tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 7
IHC testing of FFPE rat stomach tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
6 / 7
IHC testing of FFPE rat lung tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
7 / 7
IHC testing of FFPE rat small intestine tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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Western blot testing of 1) rat stomach, 2) rat lung, 3) rat NRK cells, 4) mouse heart, 5) mouse intestine and 6) mouse lung lysate with Periostin antibody at 0.5ug/ml. Predicted molecular weight ~93 kDa.
IHC testing of FFPE mouse stomach tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse lung tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse small intestine tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat stomach tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat lung tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat small intestine tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA (recombinant rat protein): 0.1-0.5ug/ml (BSA-free format available)
Application Note:
Optimal dilution of the Periostin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
POSTN (Periostin), also known as PN or OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. It was found that purified recombinant PN supported adhesion of ovarian epithelial cells. And periostin was overexpressed by the majority of human primary breast cancers examined. Transfected tumor cell lines overexpressing periostin showed accelerated growth and angiogenesis as xenografts in immunocompromised animals. It was showed that extracellular periostin induced reentry of differentiated mammalian cardiomyocytes into the cell cycle.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant rat protein corresponding to amino acids K577-Q810 was used as the immunogen for the Periostin antibody.
Limitation:
This Periostin antibody is available for research use only.
Localization:
Cytoplasm, extracellular space
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
D3ZAF5