Peroxiredoxin 4 Antibody (PRDX4)

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NSJ Bioreagents
Product Code: NSJ-R30935
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30935-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the Peroxiredoxin 4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Peroxiredoxin 4 antibody and Lane 1: rat brain; 2: (r) lung; 3: (r) liver; 4: (r) kidney; 5: human HeLa; 6: (h) 293T; 7: (h) MCF-7 cell lysate. Expected size: ~31KD
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Western blot testing of Peroxiredoxin 4 antibody and Lane 1: rat brain; 2: (r) lung; 3: (r) liver; 4: (r) kidney; 5: human HeLa; 6: (h) 293T; 7: (h) MCF-7 cell lysate. Expected size: ~31KD

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Peroxiredoxin 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Peroxiredoxin 4 is an antioxidant enzyme and belongs to the peroxiredoxin family. Functional analysis showed that PRDX4 protects glutamine synthetase from inactivation. Yeast 2-hybrid, immunoprecipitation, and immunoblot analyses indicated that PRDX4 and PRDX1 are capable of homodimerization and heterodimerization with each other but not with the mitochondrial PRDX3. Gel mobility shift and immunoblot analysis found that PRDX4 depletes NFKB binding activity together with a reduction in the amounts of p50, p65, and phosphorylated IKBA, as well as a reduction in the expression of HIV-1 viral proteins. Expression of PRDX4, alone or with PRDX1, increased the resistance of yeast cells to oxidant-induced toxicity. Jin et al.(1997) suggested Peroxiredoxin 4 modulates IKBA phosphorylation in the cytoplasm and thus affects a peroxiredoxin-dependent redox step.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human PRDX4 (SETIIPDPAGKLKYFDKLN) was used as the immunogen for this Peroxiredoxin 4 antibody (100% homologous in human, mouse and rat).
Limitation:
This Peroxiredoxin 4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13162