Peroxiredoxin 5 Antibody (PRDX5)

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NSJ Bioreagents
Product Code: NSJ-R30936
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30936-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Peroxiredoxin 5 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Peroxiredoxin 5 antibody and Lane 1: rat brain; 2: (r) lung; 3: (r) liver; 4: (r) kidney; 5: human HeLa; 6: (h) 293T; 7: (h) MCF-7; 8: (h) A549 cell lysate. Expected size: ~22KD
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IHC-P: Peroxiredoxin 5 antibody testing of human prostatic cancer tissue
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IHC-P: Peroxiredoxin 5 antibody testing of rat liver tissue
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Western blot testing of Peroxiredoxin 5 antibody and Lane 1: rat brain; 2: (r) lung; 3: (r) liver; 4: (r) kidney; 5: human HeLa; 6: (h) 293T; 7: (h) MCF-7; 8: (h) A549 cell lysate. Expected size: ~22KD
IHC-P: Peroxiredoxin 5 antibody testing of human prostatic cancer tissue
IHC-P: Peroxiredoxin 5 antibody testing of rat liver tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Peroxiredoxin 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Peroxiredoxin 5, also known as PRX5 and PRDX5, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that Peroxiredoxin 5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. Peroxiredoxin 5 plays a protective role against oxidative stress in human cartilage.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human PRDX5 (DDSLVSIFGNRRLKRF) was used as the immunogen for this Peroxiredoxin 5 antibody (100% homologous in human, mouse and rat).
Limitation:
This Peroxiredoxin 5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P30044