Peroxiredoxin 6 Antibody (PRDX6)

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NSJ Bioreagents
Product Code: NSJ-R31763
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31763-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Peroxiredoxin 6 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human placenta, 2) human PC-3, 3) human K562, 4) human HeLa, 5) rat lung, 6) rat liver, 7) mouse lung and 8) mouse liver lysate with Peroxiredoxin 6 antibody. Expected molecular weight: ~25 kDa.
2 / 7
Immunocytochemical staining of FFPE human PC-3 cells with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human stomach cancer with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human ovarian cancer with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human lung cancer with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE human breast cancer with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 7
IHC staining of FFPE rat brain with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of 1) human placenta, 2) human PC-3, 3) human K562, 4) human HeLa, 5) rat lung, 6) rat liver, 7) mouse lung and 8) mouse liver lysate with Peroxiredoxin 6 antibody. Expected molecular weight: ~25 kDa.
Immunocytochemical staining of FFPE human PC-3 cells with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human stomach cancer with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry (FFPE): 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Peroxiredoxin 6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Peroxiredoxin 6 is also known as PRX, p29 or AOP2. The protein encoded by the PRDX6 gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Peroxiredoxin 6 may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
9588
Immunogen:
Human partial recombinant protein (AA 15-224) was used as the immunogen for this Peroxiredoxin 6 antibody.
Limitation:
This Peroxiredoxin 6 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat