PF4 Antibody / Platelet factor 4

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NSJ Bioreagents
Product Code: NSJ-R32449
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32449-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Prior to reconstitution, store at 40. After reconstitution, the PF4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of mouse skeletal muslce lysate with PF4 antibody at 0.5ug/ml. Expected molecular weight: ~8/16/32 kDa (monomer/dimer/tetramer).
2 / 3
IHC testing of FFPE mouse spleen with PF4 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
3 / 3
IHC testing of FFPE rat spleen with PF4 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
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Western blot testing of mouse skeletal muslce lysate with PF4 antibody at 0.5ug/ml. Expected molecular weight: ~8/16/32 kDa (monomer/dimer/tetramer).
IHC testing of FFPE mouse spleen with PF4 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE rat spleen with PF4 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,ELISA: 0.1-0.5ug/ml (mouse protein tested); request BSA-free format for coating
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids V30-S105 were used as the immunogen for the PF4 antibody.
Limitation:
This PF4 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
Q9Z126