PGK1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31155
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31155-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the PGK1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of PGK1 antbody; Lane 1: rat liver; 2: (r) brain; 3: (r) lung; and human samples 4: A431; 5: COLO320; 6: HeLa; 7: A549; 8: Jurkat cell lysate. Expected/observed ~44KD~
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IHC-P: PGK1 antibody testing of human lung cancer tissue.
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Western blot testing of PGK1 antbody; Lane 1: rat liver; 2: (r) brain; 3: (r) lung; and human samples 4: A431; 5: COLO320; 6: HeLa; 7: A549; 8: Jurkat cell lysate. Expected/observed ~44KD~
IHC-P: PGK1 antibody testing of human lung cancer tissue.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, IHC-P: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Phosphoglycerate Kinase 1, also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. Willard et al.(1985) used a cDNA for human PGK to map the functional PGK1 gene to Xq13. Disulfide bonds in secreted proteins are considered to be inert because of the oxidizing nature of the extracellular milieu. An exception to this rule is a reductase secreted by tumor cells that reduces disulfide bonds in the serine proteinase plasmin. Lay et al.(2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. Recombinant phosphoglycerate kinase had the same specific activity as the fibrosarcoma-derived protein. Plasma of mice bearing fibrosarcoma tumors contained several-fold more phosphoglycerate kinase, as compared with mice without tumors.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human Phosphoglycerate Kinase 1 (FGTAHRAHSSMVGVN) was used as the immunogen for this PGK1 antibody (100% homologous in human, mouse and rat).
Limitation:
This PGK1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P00558