Phospholamban Antibody / PLN

Western blot: 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the Phospholamban antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Phospholamban is a 52 amino acid integral membrane protein that regulates the Ca2+ pump in cardiac muscle and skeletal muscle cells. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Phospholamban is also expressed in slow-twitch skeletal muscle and some smooth muscle cells. McTiernan et al.(1999) observed that human ventricle and quadriceps displayed high levels of phospholamban transcripts and proteins, with markedly lower expression observed in smooth muscles, while the right atrium also expressed low levels of phospholamban. The structure of the human phospholamban gene closely resembles that reported for chicken, rabbit, rat, and mouse. Comparison of the human to other mammalian phospholamban genes indicated a marked conservation of sequence for at least 217 bp upstream of the transcription start site.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the N-terminus of human Phospholamban (EKVQYLTRSAIRRAS) was used as the immunogen for this Phospholamban antibody (100% homologous in human, mouse and rat).

This Phospholamban antibody is available for research use only.

Antigen affinity

Human, Mouse, Rat

P26678