PI3K Antibody (p85 beta)

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NSJ Bioreagents
Product Code: NSJ-R30765
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30765-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PI3K antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of PI3K antibody and Lane 1: rat testis; 2: 293T; 3: HeLa cell lysate
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IHC-P: PI3K antibody testing of human breast cancer tissue
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Western blot testing of mouse 1) liver and 2) brain lysate with PI3K antibody. Predicted molecular weight ~85 kDa.
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Western blot testing of PI3K antibody and Lane 1: rat testis; 2: 293T; 3: HeLa cell lysate
IHC-P: PI3K antibody testing of human breast cancer tissue
Western blot testing of mouse 1) liver and 2) brain lysate with PI3K antibody. Predicted molecular weight ~85 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the PI3K antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Phosphatidylinositol 3-kinase regulatory subunit 2, also called p85-Beta, PIK3R2 and PIK3, is an enzyme that in humans is encoded by the PIK3R2 gene. The NS1 protein from various influenza strains bind p85-beta, but not p85-alpha. Binding of p85-beta and activation of PI3K required tyr89 of NS1, and mutant viruses expressing NS1 with a tyr89-to-phe substitution grew more slowly in cell culture than wildtype viruses. Using mouse embryonic fibroblasts, Park et al. showed that, in addition to regulating PI3K function, p85-alpha/beta regulated the function of Xbp1s, a transcription factor that orchestrates the unfolded protein response (UPR) following endoplasmic reticulum (ER) stress. Overexpression of the protein in livers of ob/ob mice increased glucose tolerance and reduced blood glucose concentrations.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 447-461 (KVYHQQYQDKSREYD-beta specific) was used as the immunogen for this PI3K antibody (100% human, mouse and rat homology).
Limitation:
This PI3K antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O00459