PKC alpha Antibody

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NSJ Bioreagents
Product Code: NSJ-R31731
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31731-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the PKC alpha antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of PKC alpha antibody and Lane 1: rat brain; 2: mouse brain; 3: (m) heart; 4: human 22RV1. Predicted/observed size ~77KD
2 / 4
IHC staining of FFPE human breast cancer with PKC alpha antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE mouse intestinal tissue with PKC alpha antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 4
IHC staining of FFPE rat intestinal tissue with PKC alpha antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of PKC alpha antibody and Lane 1: rat brain; 2: mouse brain; 3: (m) heart; 4: human 22RV1. Predicted/observed size ~77KD
IHC staining of FFPE human breast cancer with PKC alpha antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse intestinal tissue with PKC alpha antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat intestinal tissue with PKC alpha antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PKC alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Protein kinase C is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is thought to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PRKCA1 regulates cardiac contractility and propensity toward heart failure.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
5578
Immunogen:
Human partial recombinant protein (AA 153-342) was used as the immunogen for this PKC alpha antibody.
Limitation:
This PKC alpha antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat