PKC eta Antibody

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NSJ Bioreagents
Product Code: NSJ-R32029
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32029-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the PKC eta antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of human 1) A431, 2) A549, 3) HeLa and 4) SKOV lysate with PKC eta antibody. Predicted/observed molecular weight ~78 kDa.
2 / 4
Flow cytometry testing of human A431 cells with PKC eta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PKC eta antibody.
3 / 4
Flow cytometry testing of human MCF7 cells with PKC eta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PKC eta antibody.
4 / 4
Flow cytometry testing of human K562 cells with PKC eta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PKC eta antibody.
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Western blot testing of human 1) A431, 2) A549, 3) HeLa and 4) SKOV lysate with PKC eta antibody. Predicted/observed molecular weight ~78 kDa.
Flow cytometry testing of human A431 cells with PKC eta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PKC eta antibody.
Flow cytometry testing of human MCF7 cells with PKC eta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PKC eta antibody.
Flow cytometry testing of human K562 cells with PKC eta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PKC eta antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the PKC eta antibody should be determined by the researcher.
Description:
PRKCH is also known as PKC eta. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Also, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. And it is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 30-389 of human PKC eta were used as the immunogen for the PKC eta antibody.
Limitation:
This PKC eta antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P24723