PKC iota Antibody

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NSJ Bioreagents
Product Code: NSJ-R31734
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31734-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PKC iota antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of PKC iota antibody and Lane 1: SHG-44; 2: A549; 3: U87; 4: 293T; 5: HeLa; 6: Jurkat. Predicted/expected size ~68KD
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IHC-P: PKC iota antibody testing of human lung cancer tissue
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IHC-P: PKC iota antibody testing of mouse brain tissue
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IHC-P: PKC iota antibody testing of rat brain tissue
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Western blot testing of PKC iota antibody and Lane 1: SHG-44; 2: A549; 3: U87; 4: 293T; 5: HeLa; 6: Jurkat. Predicted/expected size ~68KD
IHC-P: PKC iota antibody testing of human lung cancer tissue
IHC-P: PKC iota antibody testing of mouse brain tissue
IHC-P: PKC iota antibody testing of rat brain tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PKC iota antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Protein kinase C iota, also called PKCI, is an enzyme that in humans is encoded by the PRKCI gene. This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where it phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. PRKCI is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. It is also a hedgehog target gene that forms a positive feedback loop with GLI and exists at increased levels in basal cell carcinomas.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
5584
Immunogen:
Human partial recombinant protein (AA 21-214) was used as the immunogen for this PKC iota antibody.
Limitation:
This PKC iota antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat