PKM2 Antibody
| Code | Size | Price |
|---|
| NSJ-R31156-100ug | 100 ug | £535.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the PKM2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
Documents
Further Information
Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Pyruvate Kinase, Muscle, also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate(Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the gene to 15q24-q25. Ashizawa et al.(1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al.(2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human PKM2 (SHGTHEYHAETIKNV) was used as the immunogen for this PKM2 antibody (100% homologous in human, mouse and rat).
Limitation:
This PKM2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14618